An Unusual Protector-Protégé Strategy for the Biosynthesis of Purine Nucleoside Antibiotics

development and implementation of an optimized control strategy for induction machine in an electric vehicle

in the area of automotive engineering there is a tendency to more electrification of power train. in this work control of an induction machine for the application of electric vehicle is investigated. through the changing operating point of the machine, adapting the rotor magnetization current seems to be useful to increase the machines efficiency. in the literature there are many approaches wh...

Nucleoside antibiotics: structure, biological activity, and biosynthesis.

Nucleoside antibiotics are found as diverse groups of secondary metabolites of microbial origin. They include a variety of structural modifications of nucleosides and nucleotides, often leading to intricate molecules. Their biological activities are also wide ranging, including antibacterial, antifungal, antitrypanosomal, antitumor, antiviral, herbicidal, insecticidal, immunostimulating, and of...

Effect of Purine Nucleoside Analogue-Acyclovir on The Sperm Parameters and Testosterone Production in Rats

Purine Nucleoside Phosphorylase

Purine nucleoside phosphorylase (EC 2.4.2.1) from bovine spleen is a trimeric enzyme that readily dissociates to the monomer. Dilution of enzyme from 20 to 0.02 pg of protein/ml is accompanied by a greater than 50-fold increase in the specific activity (utrimer = 0.23 nmol/min/pg; umonomer = 12.5 nmol/min/pg). Gel permeation chromatography in the presence of the substrate phosphate shows the en...

Development of an Improved Assay for Purine Nucleoside Kinase Activity

An improved assay was deyeloped to detect direct purine nucleoside phosphorylating actiyity in cell-free extracts. Direct inosine phosphorylating actiyity was detected in 2 of 70 species tested. Both actiyities, which depended oll magnesium ion and ATP, phospho"'tated a h}'droxyl group at the 5' position of inosille. The new assay was shown to be useful for screening ef direct purine nucleoside...